The enzyme linked immunosorbent assay (ELISA) is used for the detection and quantification of cytokines secreted by activated cells in culture into tissue culture supernatant. Immobilizing a cytokine-specific capture antibody onto a high protein binding capacity ELISA plate enables capture of target cytokine. The captured cytokine is detected by a biotinylated cytokine-specific antibody which recognizes a distinct epitope. The sandwiched target cytokine is quantified using a colorimetric reaction based on activity of avidin-horseradish peroxidase (bound to the biotinylated detection antibody) on a specific soluble substrate (e.g., ABTS). The colored end-product is read by a spectrophotometer.
Antibody Pairs for ELISA/Animal
Mouse Cytokine Antibody Pairs and Standards Quick Guide
