The enzyme linked immunosorbent assay (ELISA) is used for the detection and quantification of cytokines secreted by activated cells in culture into tissue culture supernatant. Immobilizing a cytokine-specific capture antibody onto a high protein binding capacity ELISA plate enables capture of target cytokine. The captured cytokine is detected by a biotinylated cytokine-specific antibody which recognizes a distinct epitope. The sandwiched target cytokine is quantified using a colorimetric reaction based on activity of avidin-horseradish peroxidase (bound to the biotinylated detection antibody) on a specific soluble substrate (e.g., ABTS). The colored end-product is read by a spectrophotometer.
Antibody Pairs for ELISA/Human
 Name Description Catalog No. Size
human Annexin V human Annexin V Module Sets - Matched Antibody Pairs ESBL2311MAP 10 plates
human anti-Annexin V human anti-Annexin V Module Sets - Matched Antibody Pairs ESBL2310MAP 10 plates
human anti-IFN-alpha human anti-IFN-alpha Module Sets - Matched Antibody Pairs ESBL2304MAP 10 plates
human APO-1/Fas human APO-1/Fas Module Sets - Matched Antibody Pairs ESBL2028MAP 10 plates
human Cathepsin L human Cathepsin L Module Sets - Matched Antibody Pairs ESBL2313MAP 10 plates
human CD152/CTLA-4 human CD152/CTLA-4 Module Sets - Matched Antibody Pairs ESBL2314MAP 10 plates
human CD28 human CD28 Module Sets - Matched Antibody Pairs ESBL2144MAP 10 plates
human CD30 human CD30 Module Sets - Matched Antibody Pairs ESBL2310MAP 10 plates
human CD40 human CD40 Module Sets - Matched Antibody Pairs ESBL2149MAP 10 plates
human CD40L human CD40L Module Sets - Matched Antibody Pairs ESBL2021MAP 10 plates
human CD44std human CD44std Module Sets - Matched Antibody Pairs ESBL2303MAP 10 plates
human CD44var (v5) human CD44var (v5) Module Sets - Matched Antibody Pairs ESBL2306MAP 10 plates
human CD44var (v6) human CD44var (v6) Module Sets - Matched Antibody Pairs ESBL2168MAP 10 plates
human Cu/ZnSOD human Cu/ZnSOD Module Sets - Matched Antibody Pairs ESBL2307MAP 10 plates
human Cytochrome c human Cytochrome c Module Sets - Matched Antibody Pairs ESBL2144MAP 10 plates
human E-selectin human E-selectin Module Sets - Matched Antibody Pairs ESBL2027MAP 10 plates
human Fas Ligand human Fas Ligand Module Sets - Matched Antibody Pairs ESBL2137MAP 10 plates
human ICAM-1 human ICAM-1 Module Sets - Matched Antibody Pairs ESBL2099MAP 10 plates
human ICAM-3 human ICAM-3 Module Sets - Matched Antibody Pairs ESBL2305MAP 10 plates
human IFN-alpha human IFN-alpha Module Sets - Matched Antibody Pairs ESBL2132MAP 10 plates
human IFN-gamma human IFN-gamma Module Sets - Matched Antibody Pairs ESBL2038MAP 10 plates
human IFN-omega human IFN-omega Module Sets - Matched Antibody Pairs ESBL2309MAP 10 plates
human IL-10 human IL-10 Module Sets - Matched Antibody Pairs ESBL2051MAP 10 plates
human IL-13 human IL-13 Module Sets - Matched Antibody Pairs ESBL2054MAP 10 plates
human IL-1alpha human IL-1alpha Module Sets - Matched Antibody Pairs ESBL2057MAP 10 plates
human IL-1beta human IL-1beta Module Sets - Matched Antibody Pairs ESBL2058MAP 10 plates
human IL-2R alpha human IL-2R alpha Module Sets - Matched Antibody Pairs ESBL2061MAP 10 plates
human IL-4 human IL-4 Module Sets - Matched Antibody Pairs ESBL2062MAP 10 plates
human IL-5 human IL-5 Module Sets - Matched Antibody Pairs ESBL2063MAP 10 plates
human IL-6 human IL-6 Module Sets - Matched Antibody Pairs ESBL2064MAP 10 plates
human IL-8 human IL-8 Module Sets - Matched Antibody Pairs ESBL2066MAP 10 plates
human LIF human LIF Module Sets - Matched Antibody Pairs ESBL2069MAP 10 plates
human LIF-R (gp190) human LIF-R (gp190) Module Sets - Matched Antibody Pairs ESBL2160MAP 10 plates
human L-selectin human L-selectin Module Sets - Matched Antibody Pairs ESBL2301MAP 10 plates
human p185HER-2 human p185HER-2 Module Sets - Matched Antibody Pairs ESBL2302MAP 10 plates
human PECAM-1 human PECAM-1 Module Sets - Matched Antibody Pairs ESBL2143MAP 10 plates
human PGL-1 human PGL-1 Module Sets - Matched Antibody Pairs ESBL2312MAP 10 plates
human TNF-alpha human TNF-alpha Module Sets - Matched Antibody Pairs ESBL2106MAP 10 plates
human TNF-beta human TNF-beta Module Sets - Matched Antibody Pairs ESBL2107MAP 10 plates
human TNF-R human TNF-R (60kDa) Module Sets - Matched Antibody Pairs ESBL2300MAP 10 plates
human TNF-R (80kDa) human TNF-R (80kDa) Module Sets - Matched Antibody Pairs ESBL2100MAP 10 plates
human VE-cadherin human VE-cadherin Module Sets - Matched Antibody Pairs ESBL2146MAP 10 plates
human VEGF-A human VEGF-A Module Sets - Matched Antibody Pairs ESBL2243MAP 10 plates
human VEGF-C human VEGF-C Module Sets - Matched Antibody Pairs ESBL2245MAP 10 plates
human VEGF-R1 human VEGF-R1 Module Sets - Matched Antibody Pairs ESBL2141MAP 10 plates
humanICAM-1 humanICAM-1 Module Sets - Matched Antibody Pairs ESBL2099MAP 10 plates

Components
   1 Capture Antibody: Pre-titrated, purified antibody
   2 Detection Antibody: Pre-titrated, biotin-conjugated antibody
   3 Standard: Recombinant cytokine for generating standard curve and calibrating samples
   4 ELISA/ELISPOT Coating Buffer Powder: Reconstitute to 1L with dH20 and filter (0.22 uM).
   5 Assay Diluent: 5X concentrated
   6 Detection enzyme: pre-titrated Avidin-HRP
   7 Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution
   8 Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards
   9 96 Well Plate: Corning Costar 9018
  USA ©2001 Ever Systems Biology Laboratory,Inc. | All rights reserved.
For Research Use Only: Not for further distribution without written consent.
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